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p ikba  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p ikba
    P Ikba, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ikba/pm41650834-91-34-36
    Average 86 stars, based on 1 article reviews
    p ikba - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Ubiquitin Proteomics:

    Article Title: Redox destabilization by ibrutinib promotes ferroptosis in diffuse large B-cell lymphoma (DLBCL).
    Article Snippet: Primary antibody species specific light chain secondary antibodies conjugate to HRP (JIM-115-035-174, JIM-112-035-175, JIM-211-032-171, Jackson ImmunoResearch) were used for signal detection on X-Ray film via a chemiluminescent substrate (NEL105001EA, PerkinElmer). .. The following antibodies were used GPX4 (Abcam, ab125066), FSP1 (Proteintech, 68049-1-Ig), GCH1 (Abnova, Cell Death Discovery (2025) 11:495 H00002643-M01), ACSL4 (Santa Cruz, sc-271800), LPCAT3 (abcam, ab232958), CD71 (Santa Cruz, sc-65882), p-P65 (CST, 3033S), IkBa (CST, 4812), total ubiquitin (Millipore, 07-375), GPX8 (ABGENT, AP16753b), GPX2 (Invitrogene, PA5-27150), TRXR1 (CST, 15140S), p-P70 (CST, #9206), cFLIP (CST, 56343), c-MYC (CST, 5605), MCL-1 (CST, 5453), xCT (abcam, ab37185), β-Actin (Sigma, A1978). .. For secondary antibodies α-rabbit IgG (211-032-171) and α-mouse IgG (115-035-174) were purchased from Jackson ImmunoResearch.

    Article Title: Redox destabilization by ibrutinib promotes ferroptosis in diffuse large B-cell lymphoma (DLBCL)
    Article Snippet: Primary antibody species specific light chain secondary antibodies conjugate to HRP (JIM-115-035-174, JIM-112-035-175, JIM-211-032-171, Jackson ImmunoResearch) were used for signal detection on X-Ray film via a chemiluminescent substrate (NEL105001EA, PerkinElmer). .. The following antibodies were used GPX4 (Abcam, ab125066), FSP1 (Proteintech, 68049-1-Ig), GCH1 (Abnova, H00002643-M01), ACSL4 (Santa Cruz, sc-271800), LPCAT3 (abcam, ab232958), CD71 (Santa Cruz, sc-65882), p-P65 (CST, 3033S), IkBa (CST, 4812), total ubiquitin (Millipore, 07-375), GPX8 (ABGENT, AP16753b), GPX2 (Invitrogene, PA5-27150), TRXR1 (CST, 15140S), p-P70 (CST, #9206), cFLIP (CST, 56343), c-MYC (CST, 5605), MCL-1 (CST, 5453), xCT (abcam, ab37185), β-Actin (Sigma, A1978). .. For secondary antibodies α-rabbit IgG (211-032-171) and α-mouse IgG (115-035-174) were purchased from Jackson ImmunoResearch.

    Western Blot:

    Article Title: TNIP1 and Autophagy Receptors regulate STING Signaling
    Article Snippet: Activation of the cGAS-STING pathway stimulates innate immune signaling as well as LC3B lipidation and ubiquitylation at Golgi-related vesicles upon STING trafficking.. Although ubiquitylation at these subcellular sites has been associated with regulating NF- B-related innate immune signaling, the mechanisms of Golgi-localized polyubiquitin chain regulation of immune signaling is not well understood.. We report here that the ubiquitinand LC3B-binding

    Incubation:

    Article Title: Hsa_circRNA_092488 Exacerbates the Progression of Deep Vein Thrombosis Through the NLRP3/NFkB Signaling Pathway.
    Article Snippet: Subsequently, the samples were transferred onto a PVDF membrane (Thermo Fisher Scientific). .. Then the membranes were blocked with 5% skimmed milk at room temperature for one hour and then incubated using primary antibodies against NLRP3 (1:1000; cat no. ab263899; Abcam), caspase-1 (1:500; cat no. ab207802; Abcam), IL-1b (1:500; cat no. ab283818; Abcam), p-NFkB-p65 (1:500; cat no. 3033; Cell Signaling Technology), NFkB-p65 (1:500; cat no. 3034; Cell Signaling Technology), p-IKBa (1:1000; cat no. 2859; Cell Signaling Technology), IKBa (1:1000; cat no. 4818; Cell Signaling Technology) and GAPDH (1:2000; cat no. 4967; Cell Signaling Technology) in a cold room overnight. .. Next day, incubation was performed using anti-mouse IgG (1:2000; cat no. ab6728; Abcam) or anti-rabbit IgG (1:2000; cat no. ab6721; Abcam) for one hour.

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway.
    Article Snippet: Then, 30 mg of each protein sample was separated through 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Merck Millipore). .. After blocking with rapid sealing fluid for 30 minutes, the membranes were incubated with primary antibodies, including COL1 (#12256; CST), RUNX2 (#8486; CST), NLRP12 (#DF14960; Affinity Biosciences), phospho-p65 (p-p65) (#3033; CST), p65 (#8242; CST), p-IkBa (#2859; CST), IkBa (#4814; CST), GAPDH (#60004; Proteintech) overnight at 4°C. .. After binding with secondary HRP-labelled Goat Anti-Rabbit IgG (H+L) (#A0208; Beyotime) or HRP-labelled Goat Anti-Mouse IgG (H+L) (#A0216; Beyotime) for 2 hours at room temperature, the protein bands were tested by the imaging system (Bio-Rad) with enhanced chemiluminescent ECL reagent (Biosharp).

    Blocking Assay:

    Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway.
    Article Snippet: Then, 30 mg of each protein sample was separated through 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Merck Millipore). .. After blocking with rapid sealing fluid for 30 minutes, the membranes were incubated with primary antibodies, including COL1 (#12256; CST), RUNX2 (#8486; CST), NLRP12 (#DF14960; Affinity Biosciences), phospho-p65 (p-p65) (#3033; CST), p65 (#8242; CST), p-IkBa (#2859; CST), IkBa (#4814; CST), GAPDH (#60004; Proteintech) overnight at 4°C. .. After binding with secondary HRP-labelled Goat Anti-Rabbit IgG (H+L) (#A0208; Beyotime) or HRP-labelled Goat Anti-Mouse IgG (H+L) (#A0216; Beyotime) for 2 hours at room temperature, the protein bands were tested by the imaging system (Bio-Rad) with enhanced chemiluminescent ECL reagent (Biosharp).



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